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cell-based luciferase reporter gene assay  (Promega)

 
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    Structured Review

    Promega cell-based luciferase reporter gene assay
    The Y150-dependence of <t>gene</t> activation of Jurkat cells: in presence of Jurkat-CD3-NFAT-RE-Luc cells only (in Green), or in presence of Jurkat-CD38 − -CD3-NFAT-RE-Luc cells only (Jurkat-4-3-F11, in Brown), or in presence of both Jurkat-CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Red), or in presence of both Jurkat- CD38 − -CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Blue). In the <t>reporter</t> gene <t>assay,</t> Jurkat cells (effector cells) and NCI-H929 (target cells) were in a ratio of 1.5:1 (E:T).
    Cell Based Luciferase Reporter Gene Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell-based luciferase reporter gene assay/product/Promega
    Average 90 stars, based on 1 article reviews
    cell-based luciferase reporter gene assay - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "Development of a reporter gene method to measure the bioactivity of anti-CD38 × CD3 bispecific antibody"

    Article Title: Development of a reporter gene method to measure the bioactivity of anti-CD38 × CD3 bispecific antibody

    Journal: Antibody Therapeutics

    doi: 10.1093/abt/tbab022

    The Y150-dependence of gene activation of Jurkat cells: in presence of Jurkat-CD3-NFAT-RE-Luc cells only (in Green), or in presence of Jurkat-CD38 − -CD3-NFAT-RE-Luc cells only (Jurkat-4-3-F11, in Brown), or in presence of both Jurkat-CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Red), or in presence of both Jurkat- CD38 − -CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Blue). In the reporter gene assay, Jurkat cells (effector cells) and NCI-H929 (target cells) were in a ratio of 1.5:1 (E:T).
    Figure Legend Snippet: The Y150-dependence of gene activation of Jurkat cells: in presence of Jurkat-CD3-NFAT-RE-Luc cells only (in Green), or in presence of Jurkat-CD38 − -CD3-NFAT-RE-Luc cells only (Jurkat-4-3-F11, in Brown), or in presence of both Jurkat-CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Red), or in presence of both Jurkat- CD38 − -CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Blue). In the reporter gene assay, Jurkat cells (effector cells) and NCI-H929 (target cells) were in a ratio of 1.5:1 (E:T).

    Techniques Used: Activation Assay, Reporter Gene Assay

    Validation of reporter gene assay. (A) Specificity assessment of the assay. Y150 or a nonspecific antibody was incubated with both NCI-H929 cells and Jurkat T cell line 4-3-F11, and the RLU signals were measured. Y150 sample stressed under oxidization with 4% hydrogen peroxide was tested and compared with Y150 reference sample. (B) Analysis of linearity of the assay was conducted by comparing the measured potency and expected potency. Each point represents the mean of six independent experiments.
    Figure Legend Snippet: Validation of reporter gene assay. (A) Specificity assessment of the assay. Y150 or a nonspecific antibody was incubated with both NCI-H929 cells and Jurkat T cell line 4-3-F11, and the RLU signals were measured. Y150 sample stressed under oxidization with 4% hydrogen peroxide was tested and compared with Y150 reference sample. (B) Analysis of linearity of the assay was conducted by comparing the measured potency and expected potency. Each point represents the mean of six independent experiments.

    Techniques Used: Reporter Gene Assay, Incubation

    The correlations of reporter gene assay results with those from cytotoxicity assay and sandwich ELISA assay
    Figure Legend Snippet: The correlations of reporter gene assay results with those from cytotoxicity assay and sandwich ELISA assay

    Techniques Used: Reporter Gene Assay, Cytotoxicity Assay, Sandwich ELISA



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    The Y150-dependence of <t>gene</t> activation of Jurkat cells: in presence of Jurkat-CD3-NFAT-RE-Luc cells only (in Green), or in presence of Jurkat-CD38 − -CD3-NFAT-RE-Luc cells only (Jurkat-4-3-F11, in Brown), or in presence of both Jurkat-CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Red), or in presence of both Jurkat- CD38 − -CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Blue). In the <t>reporter</t> gene <t>assay,</t> Jurkat cells (effector cells) and NCI-H929 (target cells) were in a ratio of 1.5:1 (E:T).
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    The Y150-dependence of <t>gene</t> activation of Jurkat cells: in presence of Jurkat-CD3-NFAT-RE-Luc cells only (in Green), or in presence of Jurkat-CD38 − -CD3-NFAT-RE-Luc cells only (Jurkat-4-3-F11, in Brown), or in presence of both Jurkat-CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Red), or in presence of both Jurkat- CD38 − -CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Blue). In the <t>reporter</t> gene <t>assay,</t> Jurkat cells (effector cells) and NCI-H929 (target cells) were in a ratio of 1.5:1 (E:T).
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    The Y150-dependence of <t>gene</t> activation of Jurkat cells: in presence of Jurkat-CD3-NFAT-RE-Luc cells only (in Green), or in presence of Jurkat-CD38 − -CD3-NFAT-RE-Luc cells only (Jurkat-4-3-F11, in Brown), or in presence of both Jurkat-CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Red), or in presence of both Jurkat- CD38 − -CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Blue). In the <t>reporter</t> gene <t>assay,</t> Jurkat cells (effector cells) and NCI-H929 (target cells) were in a ratio of 1.5:1 (E:T).
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    Image Search Results


    The Y150-dependence of gene activation of Jurkat cells: in presence of Jurkat-CD3-NFAT-RE-Luc cells only (in Green), or in presence of Jurkat-CD38 − -CD3-NFAT-RE-Luc cells only (Jurkat-4-3-F11, in Brown), or in presence of both Jurkat-CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Red), or in presence of both Jurkat- CD38 − -CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Blue). In the reporter gene assay, Jurkat cells (effector cells) and NCI-H929 (target cells) were in a ratio of 1.5:1 (E:T).

    Journal: Antibody Therapeutics

    Article Title: Development of a reporter gene method to measure the bioactivity of anti-CD38 × CD3 bispecific antibody

    doi: 10.1093/abt/tbab022

    Figure Lengend Snippet: The Y150-dependence of gene activation of Jurkat cells: in presence of Jurkat-CD3-NFAT-RE-Luc cells only (in Green), or in presence of Jurkat-CD38 − -CD3-NFAT-RE-Luc cells only (Jurkat-4-3-F11, in Brown), or in presence of both Jurkat-CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Red), or in presence of both Jurkat- CD38 − -CD3-NFAT-RE-Luc cells and NCI-H929 cells (in Blue). In the reporter gene assay, Jurkat cells (effector cells) and NCI-H929 (target cells) were in a ratio of 1.5:1 (E:T).

    Article Snippet: Promega Corporation developed a cell-based luciferase reporter gene assay to monitor the Jurkat T cell activation through binding to CD3 on the Jurkat T cells engineered with an nuclear factor of activated T cells response element (NFAT-RE) driving luciferase expression [ , ].

    Techniques: Activation Assay, Reporter Gene Assay

    Validation of reporter gene assay. (A) Specificity assessment of the assay. Y150 or a nonspecific antibody was incubated with both NCI-H929 cells and Jurkat T cell line 4-3-F11, and the RLU signals were measured. Y150 sample stressed under oxidization with 4% hydrogen peroxide was tested and compared with Y150 reference sample. (B) Analysis of linearity of the assay was conducted by comparing the measured potency and expected potency. Each point represents the mean of six independent experiments.

    Journal: Antibody Therapeutics

    Article Title: Development of a reporter gene method to measure the bioactivity of anti-CD38 × CD3 bispecific antibody

    doi: 10.1093/abt/tbab022

    Figure Lengend Snippet: Validation of reporter gene assay. (A) Specificity assessment of the assay. Y150 or a nonspecific antibody was incubated with both NCI-H929 cells and Jurkat T cell line 4-3-F11, and the RLU signals were measured. Y150 sample stressed under oxidization with 4% hydrogen peroxide was tested and compared with Y150 reference sample. (B) Analysis of linearity of the assay was conducted by comparing the measured potency and expected potency. Each point represents the mean of six independent experiments.

    Article Snippet: Promega Corporation developed a cell-based luciferase reporter gene assay to monitor the Jurkat T cell activation through binding to CD3 on the Jurkat T cells engineered with an nuclear factor of activated T cells response element (NFAT-RE) driving luciferase expression [ , ].

    Techniques: Reporter Gene Assay, Incubation

    The correlations of reporter gene assay results with those from cytotoxicity assay and sandwich ELISA assay

    Journal: Antibody Therapeutics

    Article Title: Development of a reporter gene method to measure the bioactivity of anti-CD38 × CD3 bispecific antibody

    doi: 10.1093/abt/tbab022

    Figure Lengend Snippet: The correlations of reporter gene assay results with those from cytotoxicity assay and sandwich ELISA assay

    Article Snippet: Promega Corporation developed a cell-based luciferase reporter gene assay to monitor the Jurkat T cell activation through binding to CD3 on the Jurkat T cells engineered with an nuclear factor of activated T cells response element (NFAT-RE) driving luciferase expression [ , ].

    Techniques: Reporter Gene Assay, Cytotoxicity Assay, Sandwich ELISA